Growth of B-lymphocyte colonies in vitro.

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Growth of B - Lymphocyte Colonies in Vitro

Semisolid culture systems have been developed which permit the proliferation of mousehemopoietic progenitor cells to form colonies of neutrophils and macrophages (1, 2), erythropoietic cells (3), eosinophils (4), and megakaryocytes (5) . These cloning systems have made it possible to analyse in detail many aspects of the nature and control of these hemopoietic populations . With a cloning syste...

متن کامل

Growth of B-lymphocyte colonies in vitro

In semisolid agar cultures containing mercaptoethanol, cells from the spleen, lymph nodes, marrow, peritoneal cavity, thoracic duct, and blood of normal mice generated clusters and colonies of up to 3,000 cells. Colony numbers and growth were markedly enhanced by the addition of sheep red cells. The frequency of colony-forming cells in the spleen or lymph nodes was 0.5-2.0%, and cluster forming...

متن کامل

Congenital neutropenia: in vitro growth of colonies mimicking the disease.

Congenital neutropenia is a lethal disease characterized by recurrent infections beginning in the neonatal period, absence of neutrophils in the peripheral blood, eosinophilia, and monocytosis. The bone marrow shows an apparent "maturation arrest" of the neutrophil series at the promyelocyte stage. Granulocytic colonies grown in vitro in soft agar medium show normal development of eosinophilic ...

متن کامل

Growth and detection of human bone marrow B-lineage colonies.

A reproducible method for growing normal human bone marrow B-lineage colonies in agar is described. The clonogenic cells require a rich medium, Opti-MEM (GIBCO/BRL, Burlington, Ontario, Canada), and a source of T-cell-derived factors for growth. The conditions described appear to be limiting for the colony progenitor, suggesting assay clonality. Three novel methods that permit routine and rapid...

متن کامل

In vitro growth of myeloid colonies from bone marrow of patients with acute leukemia in remission.

By JULEs HARRIS AND EMIL J. FREIREICH T HE TREATMENT OF LEUKEMIA is guided by histologic examination of bone marrow specimens. Morphologic criteria alone may be insufficient for determining whether therapy has produced remission of disease. For cxample, in chronic myelogenous leukemia the marrow may appear morphologically normal but cytogenetic examination of bone marrow samples will show the p...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Experimental Medicine

سال: 1975

ISSN: 0022-1007,1540-9538

DOI: 10.1084/jem.142.6.1534